pdh  (Cell Signaling Technology Inc)

Journal: Cardiovascular Diabetology

Article Title: Distinct immunometabolic signatures of type 1 versus type 2 diabetes in a murine model of myocardial infarction

doi: 10.1186/s12933-026-03099-y

Figure Lengend Snippet: Markers of glucose, fatty acid, and ketone metabolism in the remote LV. A Expression of genes associated with glucose metabolism. B Western blot analysis of phosphorylated and total levels of PDH and Akt. C Expression of genes associated with fatty acid and ketone metabolism. D Western blot analysis of phosphorylated and total levels of ACC. * p < 0.05 versus D0 control (Ctrl), # p < 0.05 versus Ctrl from same time point

Article Snippet: Membranes were then blocked for 1 h (SuperBlockTM Blocking Buffer, Thermo Scientific), and incubated with primary antibodies against PDH (1:1000; CST #3205S), phospho-PDH (Serine 293; 1:1000; CST #31866S), phospho-Akt (serine 473; 1:1000; Cell Signaling Technologies #9271), Akt (1:1000; CST #9272), phospho-ACC (serine 79; 1:1000; CST #D7D11), and ACC (1:1000; CST #3676).

Techniques: Expressing, Western Blot, Control